Thymidine phosphorylase expression in patients with chronic viral hepatitis
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K.Mimidis, A.Giatromanolaki, E.Sivridis, V.Papadopoulos, K.Ritis, G.Kartalis

1) First Department of Internal Medicine. 2) Department of Pathology, Democritus University of Thrace,Alexandroupolis, Greece

Abstract

Background. The role of thymidine phosphorylase (TP), an angiogenic factor, in chronic viral hepatitis is unclear. In the present study we investigated TP expression in chronic viral hepatitis due to hepatitis B virus (HBV) and hepatitis C virus (HCV) infection.

Methodology. Fifty-eight patients suffering from chronic viral hepatitis and 13 cirrhotic patients due to either HBV or HCV were studied. TP expression was investigated using a standard immunohistochemical technique and the specific antibody P-GF.44C.

Results. The pattern of TP staining was predominantly nuclear, or mixed nuclear/cytoplasmic, but no significant difference in such expression was noted between chronic hepatitis B and C: nuclear immunostaining, t=0.0616, p=0.9511; cytoplasmic immunostaining, t=0.5188, p=0.6060. There was, however, a marked difference in TP expression between patients with chronic hepatitis B/C and cirrhosis. Notably, this finding applied to both nuclear (p=0.00754, p=0.0143, respectively) and cytoplasmic (p=0.000091, p=0.000031, respectively) expression.

Conclusion. These observations indicate that TP expres-sion, although linked to liver cirrhosis, may not be virus-related.

Key words

Thymidine phosphorylase - chronic viral hepatitis - cirrhosis