Denervation Influence on Hepatocellular Oxidative Stress in
Brain Death and Living Donors*
Octavian Bud1,2, M. Golling1, H. Kellner2, Th. Kraus1, M.M.
Gebhard2, Christian Herfarth1 and E. Klar1
Aim of the study. Liver function following
brain death (BD) is mainly influenced via central action of
sympathetic nerves and changes in systemic and portal perfusion.
We examined the specific influence of surgical denervation on
the intrahepatic oxidative stress (reduced [rGSHL] and oxidised
[GSSGL] glutathione) in cardiocirculatory stable BD animals
compared to living donors [LD] in a porcine model.
Methods. BD (n=8) was induced via continuous NaCl inflation
of an epidurally placed Tieman-catheter (1 mL/5 min) and compared
to the control group (n=6) of living donors. Measurements of
the hepatic microperfusion (thermodif-fusion probe: ml/100g/min),
and the glutathione [rGSHL, GSSGL ; nmol/mg protein] were performed
2 h following BD and 2 h following denervation of the liver.
For statistical analysis the Wilcoxon and Mann-Witney U-Test
was used. Overall significance was set at p<0.05.
Results. In comparison with LD, BD animals showed a
reduced rGSHL (19±1.5 [BD] vs. 32±1 [LD]; p<0.01];
GSSGL (1.7±0.4 [BD] vs. 0.7±0.5 [LD]; p<0.01)
and a severe reduced redox potential (GSHL/GSSGL: 11.5±1.0[BD]
vs. 43.1±0.08[LD]; p<0.001). Surgical denervation
resulted in reduction of oxidative stress in the BD group [BD:
rGSHL:31±1 (p<0.01); GSSGL:0.96±0.02 (p<0.05);
GSHL/GSSGL:31.3±0.006 (p<0.01)] while it increased
in the living donors [LD: rGSHL:15±0.7 (p<0.01); GSSGL:
1.1 ± 0.02 (p<0.05); GSHL/GSSGL: 13.8±0.05
Conclusion. Intrahepatic oxidative stress is increased
in cardiocirculatory stable BD animals compared with LD. Surgical
denervation of the liver will reduce the oxidative stress only
in BD animals while it increases in living donors.
The results imply a potential benefit of surgical denervation
of the liver before explantation in BD donors only.
Liver transplantation - oxidative stress _ brain death _ living