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ORIGINAL PAPER

A Multi-strain Probiotic Reduces the Fatty Liver Index, Cytokines and Aminotransferase levels in NAFLD Patients: Evidence from a Randomized Clinical Trial

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Nazarii Kobyliak1, Ludovico Abenavoli2, Galyna Mykhalchyshyn1, Liudmyla Kononenko1, Luigi Boccuto3, Dmytro
Kyriienko1,4, Oleg Dynnyk5

1) Department of Endocrinology, Bogomolets National Medical University, Kiev, Ukraine;
2) Department of Health Sciences, University Magna Graecia of Catanzaro, Italy;
3) Research Department, Greenwood Genetic Center, School of Health Research, Clemson University, Greenwood 29646, United States
4) Kyiv City Clinical Endocrinology Center, Kiev, Ukraine
5) Bogomolets Institute of Physiology of the Ukrainian National Academy of Science, Kiev, Ukraine

DOI: http://dx.doi.org/10.15403/jgld.2014.1121.271.kby

ABSTRACT
Background:
Probiotics have a beneficial effect on nonalcoholic fatty liver disease (NAFLD) in animal models. Randomized placebo-controlled trials (RCTs) in NAFLD are still lacking in humans despite a large number of data from animal research.
Aim: We performed a double-blind single center RCT of live multi-strain probiotic vs. placebo in type 2 diabetes patients with NAFLD.
Methods: A total of 58 patients met the criteria for inclusion. They were randomly assigned to receive the multiprobiotic “Symbiter” (concentrated biomass of 14 probiotic bacteria genera Bifidobacterium, Lactobacillus, Lactococcus, Propionibacterium) or placebo for 8-weeks administered as a sachet formulation in double-blind treatment. The primary main outcomes were the changes in fatty liver index (FLI) and liver stiffness (LS) measured by Shear Wave Elastography (SWE). Secondary outcomes were the changes in aminotransferase activity, serum lipids and cytokines (TNF-α, IL-1β, IL-6, IL-8, and IFN-γ) levels. Analysis of covariance was used to assess the difference between groups.
Results: In the probiotic group, FLI significantly decreased from 84.33±2.23 to 78.73±2.58 (p<0.001) but it did not change in the placebo group (82.57±2.45 to 81.6 ±2.36; p=0.367). In both groups a slight but not significant reduction of LS measured by SWE was detected. Analysis of the secondary outcomes showed that probiotics reduced the level of serum AST and GGT. Among the markers of chronic systemic inflammatory state, only TNF-α and IL-6 levels changed significantly after the treatment with the probiotic.
Conclusion: The probiotic “Symbiter” reduces liver fat, aminotransferase activity, and the TNF-α and IL-6 levels in NAFLD patients. Modulation of the gut microbiota might represent a new therapy for NAFLD, which should be tested in larger studies.
Key words: probiotics − NAFLD − cytokine − aminotransferase − fatty liver index − liver stiffness.
Abbreviations: ALT: alanine aminotransferase; AST: aspartate aminotransferase; BMI: body mass index; CFU: colony-forming unit; FLI: fatty liver index; GGT: gamma glutamyl transferase; HDL-C: high-density lipoprotein cholesterol; LDL-C: low-density lipoprotein cholesterol; MSG: mono-sodiume glutamate; NAFLD: non-alcoholic fatty liver disease; NASH: non-alcoholic steatohepatitis; RCT: randomized placebo-controlled trials; SCFAs: short-chain fatty acids; SU: sulfonylurea; SWE: Shear Wave Elastography; T2D: type 2 diabetes; TC: total cholesterol; TG: triglyceride; TNF-α: tumor necrosis factor; US: ultrasonography; WC: waist circumference.