Tissue and Circulating MicroRNA-31, MicroRNA-200b, and MicroRNA-200c Reflects Disease Activity in Crohn‘s Disease Patients: Results from the BIOMIR Study

Authors

  • Cristina Tocia Ovidius University of Constanta, Faculty of Medicine, Constanta; Gastroenterology Clinic, County Clinical Emergency Hospital of Constanta, Constanta, Romania
  • Andrei Dumitru Ovidius University of Constanta, Faculty of Medicine, Constanta; Gastroenterology Clinic, County Clinical Emergency Hospital of Constanta, Constanta, Romania
  • Bogdan Mateescu Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
  • Lucian Negreanu Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
  • Monica State Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
  • Georgeta Camelia Cozaru Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology (CEDMOG), Ovidius University of Constanta, Constanta; Clinical Service of Pathology, County Clinical Emergency Hospital of Constanta, Constanta, Romania
  • Anca Florentina Mitroi Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology (CEDMOG), Ovidius University of Constanta, Constanta; Clinical Service of Pathology, County Clinical Emergency Hospital of Constanta, Constanta, Romania
  • Costel Brinzan Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology (CEDMOG), Ovidius University of Constanta, Constanta; Clinical Service of Pathology, County Clinical Emergency Hospital of Constanta, Constanta, Romania
  • Razvan Popescu Ovidius University of Constanta, Faculty of Medicine, Constanta, Romania
  • Nicoleta Leopa Ovidius University of Constanta, Faculty of Medicine, Constanta, Romania
  • Miorita Melina Iordache Ovidius University of Constanta, Faculty of Medicine, Constanta, Romania
  • Mihaela Manea Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology (CEDMOG), Ovidius University of Constanta, Constanta, Romania
  • Elena Matei Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology (CEDMOG), Ovidius University of Constanta, Constanta, Romania
  • Eugen Dumitru Ovidius University of Constanta, Faculty of Medicine, Constanta; Gastroenterology Clinic, County Clinical Emergency Hospital of Constanta, Constanta; Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology (CEDMOG), Ovidius University of Constanta, Constanta, Romania
  • Luana Alexandrescu Ovidius University of Constanta, Faculty of Medicine

DOI:

https://doi.org/10.15403/jgld-4656

Keywords:

microRNA, disease activity, Crohn's disease, fecal calprotectin, biomarkers

Abstract

Background and Aims: MicroRNAs (miR) have altered expression in multiple autoimmune disorders including inflammatory bowel disease. The aim of the study was to assess the tissue and circulating miR-31, miR-200b, and miR-200c expression levels as potential biomarkers for intestinal disease activity in patients with Crohn’s disease (CD).

Methods: The study included 45 patients with histopathological confirmed CD and active disease (defined as fecal calprotectin >50 μg/g and Simple Endoscopic Score (SES) of CD >3), and 21 subjects as controls for the validation cohort. Demographic and clinical data, biomarkers (fecal calprotectin), endoscopy data, the expression levels of miR-31, miR-200b, and miR-200c in tissue and serum were assessed (by RT-PCR). Receiver operating characteristic analysis was performed to assess the miR-31, miR-200b, and miR-200c expression levels as potential biomarkers for active CD.

Results: Mean fecal calprotectin was 1540±890 μg/g. Mean SES-CD was 8.9±4.2. Tissue and circulating miR- 31 were significantly correlated with fecal calprotectin (r=0.81, r=0.83, p<0.01) and with SES-CD (r=0.82, r=0.79, p<0.01). The expression level of miR-31 was significantly upregulated in CD tissue cases compared to the control tissue samples (6.24±1.57 vs. 3.70±1.44; p <0.01). Similarly, serum miR-31 expression levels in CD patients were significantly upregulated compared to the control serum samples (0.78±0.42 vs. -2.07±1.00; p<0.01). The expression levels of tissue miR-200b and miR-200c were significantly upregulated in CD tissue cases compared to the control tissue samples (-5.25±0.93 vs. -4.69±0.80, p=0.03 for miR-200b, and -0.86±0.96 vs. 0.39±0.66, p<0.01 for miR-200c). Similarly, serum miR-200b and miR-200c expression levels in CD patients were significantly upregulated compared to the control serum samples (p < 0.05). Receiver operating characteristic analysis revealed that the expression levels of the selected miRNAs could help to discriminate active CD patients from healthy controls with very good specificity and sensitivity.

Conclusions: Tissue and circulating miR-31, miR-200b, and miR-200c reflect disease activity in CD patients and can be used as biomarkers for active disease.

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Published

2023-03-31

How to Cite

1.
Tocia C, Dumitru A, Mateescu B, Negreanu L, State M, Cozaru GC, Mitroi AF, Brinzan C, Popescu R, Leopa N, Iordache MM, Manea M, Matei E, Dumitru E, Alexandrescu L. Tissue and Circulating MicroRNA-31, MicroRNA-200b, and MicroRNA-200c Reflects Disease Activity in Crohn‘s Disease Patients: Results from the BIOMIR Study. JGLD [Internet]. 2023 Mar. 31 [cited 2025 Jun. 15];32(1):30-8. Available from: https://www.jgld.ro/jgld/index.php/jgld/article/view/4656

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Original Article